spec protein ladders for western blotting

SPEC PROTEIN LADDERS FOR WESTERN BLOTTING PLUS

Next, the proteins are transferred from the gel to a membrane. These standards contain ten Strep-tagged recombinant proteins (10250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

SPEC PROTEIN LADDERS FOR WESTERN BLOTTING PLUS

NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). First, proteins are separated from each other based on their size by SDS-PAGE gel electrophoresis. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. Western blotting (WB) analysis is the most popular and widely used methodology for protein detection and characterization over recent decades. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. In SDS-PAGE it allows for the monitoring of protein migration, as. c) ab116029 for smaller proteins down to 3.5 kDa.

b) ab116028 for larger proteins up to 245 kDa. For most researchers, we generally recommend: a) ab116027 for proteins between 10 and 180 kDa. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. They are commonly used in both SDS-polyacrylamide gel electrophoresis and western blotting. Each vial contains five individually colored proteins, allowing instant band recognition on western blots or SDS-PAGE gels. When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest.

Spec protein ladders for western blotting